Journal: Methods in molecular biology (Clifton, N.J.)
Article Title: Analysis of Vascular Hydrogen Sulfide Biosynthesis
doi: 10.1007/978-1-4939-9528-8_3
Figure Lengend Snippet: Semiquantitative assessment and localization of CBS and CSE. UA sections were labeled with antibodies against CBS, or CSE (not shown), followed by secondary Alexa488 (green)-labeled secondary antibody. Endothelial cells were labeled with CD31 followed by Alexa568 (red)-labeled secondary antibody. Cell nuclei were stained with DAPI. Negative control treated with IgG or with primary antibody omitted is shown in first panel. Representative outline of border between UA intima and media was indicated, and UA lumen (l), intima (i), and media (m) were denoted (second panel). Regions of interest for intima (ROI – i) and media (ROI – m) used for analysis in Simple PCI software are illustrated (third panel). Scale bar is 25 μm
Article Snippet: Image Analysis list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Open image in Simple PCI Image analysis software (Hamamatsu Corp, Sewickley, PA, USA).
Techniques: Labeling, Staining, Negative Control, Software